1. Constitution of the column The column is the core component for separation, requiring high column efficiency, large column capacity, and stable performance. Column performance is related to column structure, filler properties, fill quality, and service conditions.
Chromatographic Packing: After preparation, the particles used to fill the column are usually spherical particles with a particle size of 5-10.
Column tube: internally polished stainless steel tube. A typical liquid chromatography column size is 4.6 mm ID and 250 mm long.
Columns: Also known as stationary phases, chromatographic packings are filled into column tubes.
2. Fill the column with a dry filling method: Place a soft pad on the hard countertop, place the top of the empty column tube vertically on a soft pad, fill the funnel with 50-100 mg of filler each time, and then vertical the table pier 10-20 times .
Wet-filling: Also known as slurry filling, special filling devices are used.
3. Structure of the packing Chromatographic packing consists of two parts: the matrix and the functional layer.
Matrix: Often referred to as carrier or carrier, it is usually prepared as a spherical particle with a particle size of several micrometers to several tens of micrometers. It has a certain rigidity, can withstand a certain pressure, does not have a significant effect on separation, but only as a functional group. Carrier. Silica gel and organic macromolecule polymer microspheres are commonly used as substrates.
Functional layer: An organic molecule or functional group that is fixed on the surface of a substrate by chemical or physical means and plays a substantial role in the retention of sample molecules. A schematic diagram of the structure of the crown ether macromolecular stationary phase of the silica matrix, the functional layer crown ether molecule is adsorbed or bonded to the surface of the silica gel matrix.
The physical structure of the filler: divided into four types: microporous (or gel), macroporous (fully porous), thin shell, and superficial porous
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