Tumorous stem mustard leaves are rich in chlorophyll. Chlorophyll is the most promising natural green pigment. Chlorophyll and its derivatives have the function of improving physiological activities. They are widely used in the food industry, but many factors such as temperature, light and oxygen will affect Chlorophyll stability. The determination of chlorophyll content in stem tumor mustard by using chlorophyll meter and its spectral characteristics and various factors affecting the stability of chlorophyll were studied to provide reference for the further development and utilization of tumorous stem mustard.
In addition to measuring the chlorophyll content using the chlorophyll meter, there is also a certain characteristic for the absorption of light. A certain amount of chlorophyll acetone solution was taken and wavelength scanning was performed in the visible light region of 400 to 760 nm. The chlorophyll-acetone solution from the stem mustard leaves had two absorption peaks in the visible light region, one of which was at the 655nm wavelength in the red portion and the other was at the 420nm wavelength in the blue-violet region. The chlorophylls of higher plants are classified into chlorophyll a and chlorophyll b, and their absorption spectra overlap each other in the visible light region.
A certain amount of chlorophyll-acetone solution was taken and treated with different conditions. The fresh chlorophyll-acetone solution was used as a control to perform wavelength scanning in the visible light region of 400-760 nm, and the chlorophyll meter was used to study the stability of chlorophyll. Chlorophyll is very sensitive to light. Visible light has a degrading effect on chlorophyll. The greater the light intensity, the faster the chlorophyll degradation and the oxygen accelerates the degradation of chlorophyll. Under indoor natural light, the chlorophyll of the stem mustard leaves was preserved for 5 hours. The chlorophyll absorbance of the stem mustard leaves had almost no change.
Chlorophyll meter determination of its content Sampling study of the chlorophyll of leaf tumor mustard in the outdoor natural light, with the increase of light time, chlorophyll absorbance decreased significantly, to 70min, the chlorophyll absorption peak disappeared in the stem mustard leaf. The absorbance of chlorophyll-acetone solution in stem mustard leaves at 10～70°C had almost no change, and the solution of chlorophyll-acetone was used to treat the shoot mustard at 80°C. The absorbance of chlorophyll in the tumor mustard at 655nm and 420nm was reduced.
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